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battery operated spinning homogenizer  (Bio-Rad)


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    Structured Review

    Bio-Rad battery operated spinning homogenizer
    Battery Operated Spinning Homogenizer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/battery+operated+spinning+homogenizer/Battery/pmc04499058-309-97-94
    Average 93 stars, based on 13 article reviews
    battery operated spinning homogenizer - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Spectrophotometry:

    Article Title: A 3D human neural cell culture system for modeling Alzheimer's disease.
    Article Snippet: .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free microcentrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 thermal cycler (MJ Research, cat. no. ALD-1244) iCycler iQ (Bio-Rad, cat. no. 170-8740) Bio-Rad Tetrad 2 thermal cycler (Bio-Rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-Rad ChemiDoc XRS system (Bio-Rad, cat. no. 170-8070) IF and immunohistochemistry Olympus IX7 fluorescence microscope equipped with a spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical extraction and western blotting Hoefer semi-dry transfer unit (Hoefer, TE77) Molecular imager (Bio-Rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) • • • • • • • • • • • • • • • • • • • • • • • • Ultracentrifuge (Beckman, Optima TL ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) REAGENT SETUP Lentivirus We obtained the lentiviral polycistronic vectors (CSCW-IRES-GFP and CSCW-IRES-mCherry) from the Massachusetts General Hospital (MGH) viral core42, and these are available through the MGH viral core (https://vectorcore.mgh.harvard.edu). ..

    Article Title: A 3D human neural cell culture system for modeling Alzheimer’s disease
    Article Snippet: 0.4 μm disposable cell culture medium filter (Life Technologies, cat. no. 353095) Sterile 15 and 50 ml conical tube (Fisher Scientific/Falcon, cat. no. 352099 and 352070) Automated cell counter (Bio-rad, cat. no. TC10) Cell counter slide (Bio-rad, cat. no. 1450011) Water Bath (VWR Scientific, cat. no. 1202) Micro-centrifuge, 4 °C (Beckman Coulter, cat. no. 367160) Tissue culture Light microscope (Zeiss) BL-2 biosafety culture hoods Vortex Sorvall T6000B Centrifuge (Brochu) RNA extraction and RT-PCR Sorvall Legend T+ centrifuge (Thermo Scientific, cat. no. 75004367) RNaseZap®-rinsed, sterile forceps ▲ CRITICAL Forceps should be dedicated for RNA work only and stored in a separate RNA working area RNaseZap ® -rinsed, disposable razor blades RNase-free filter pipette tips (1, 20, 100, 1,000 μl; USA Scientific, cat. nos. .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free micro-centrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 Thermal Cycler (MJ Research, cat. no. ALD-1244) iCycler iQ TM (Bio-rad, cat. no. 170-8740) Bio-rad Tetrad® 2 Thermal Cycler (Bio-rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 Spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-rad ChemiDoc XRS System (Bio-rad, cat. no. 170-8070) Immunofluorescence and Immunohistochemistry Olympus IX7 fluorescence microscope equipped with spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical Extraction and Western Blot Hoefer Semi-Dry Transfer Unit (Hoefer, TE77) Molecular imager (Bio-rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) Ultracentrifuge (Beckman, Optima TL Ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader) .. We use a BD SORP FACSAria2 cell sorter (BD Bioscience) in a Bioprotect IV biosafety cabinet (Baker Company): equipped with 5 lasers (350, 405, 488, 561 and 640), located at the Imaging Core Facility of the Ragon Institute of MGH/MIT/Harvard ( http://ragoninstitute.org/research/services/flow-cytometry/ ) Cell strainer filter (70 μm Nylon, Corning/Fisher Scientific, cat. no. 352350)

    Immunohistochemistry:

    Article Title: A 3D human neural cell culture system for modeling Alzheimer's disease.
    Article Snippet: .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free microcentrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 thermal cycler (MJ Research, cat. no. ALD-1244) iCycler iQ (Bio-Rad, cat. no. 170-8740) Bio-Rad Tetrad 2 thermal cycler (Bio-Rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-Rad ChemiDoc XRS system (Bio-Rad, cat. no. 170-8070) IF and immunohistochemistry Olympus IX7 fluorescence microscope equipped with a spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical extraction and western blotting Hoefer semi-dry transfer unit (Hoefer, TE77) Molecular imager (Bio-Rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) • • • • • • • • • • • • • • • • • • • • • • • • Ultracentrifuge (Beckman, Optima TL ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) REAGENT SETUP Lentivirus We obtained the lentiviral polycistronic vectors (CSCW-IRES-GFP and CSCW-IRES-mCherry) from the Massachusetts General Hospital (MGH) viral core42, and these are available through the MGH viral core (https://vectorcore.mgh.harvard.edu). ..

    Article Title: A 3D human neural cell culture system for modeling Alzheimer’s disease
    Article Snippet: 0.4 μm disposable cell culture medium filter (Life Technologies, cat. no. 353095) Sterile 15 and 50 ml conical tube (Fisher Scientific/Falcon, cat. no. 352099 and 352070) Automated cell counter (Bio-rad, cat. no. TC10) Cell counter slide (Bio-rad, cat. no. 1450011) Water Bath (VWR Scientific, cat. no. 1202) Micro-centrifuge, 4 °C (Beckman Coulter, cat. no. 367160) Tissue culture Light microscope (Zeiss) BL-2 biosafety culture hoods Vortex Sorvall T6000B Centrifuge (Brochu) RNA extraction and RT-PCR Sorvall Legend T+ centrifuge (Thermo Scientific, cat. no. 75004367) RNaseZap®-rinsed, sterile forceps ▲ CRITICAL Forceps should be dedicated for RNA work only and stored in a separate RNA working area RNaseZap ® -rinsed, disposable razor blades RNase-free filter pipette tips (1, 20, 100, 1,000 μl; USA Scientific, cat. nos. .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free micro-centrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 Thermal Cycler (MJ Research, cat. no. ALD-1244) iCycler iQ TM (Bio-rad, cat. no. 170-8740) Bio-rad Tetrad® 2 Thermal Cycler (Bio-rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 Spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-rad ChemiDoc XRS System (Bio-rad, cat. no. 170-8070) Immunofluorescence and Immunohistochemistry Olympus IX7 fluorescence microscope equipped with spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical Extraction and Western Blot Hoefer Semi-Dry Transfer Unit (Hoefer, TE77) Molecular imager (Bio-rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) Ultracentrifuge (Beckman, Optima TL Ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader) .. We use a BD SORP FACSAria2 cell sorter (BD Bioscience) in a Bioprotect IV biosafety cabinet (Baker Company): equipped with 5 lasers (350, 405, 488, 561 and 640), located at the Imaging Core Facility of the Ragon Institute of MGH/MIT/Harvard ( http://ragoninstitute.org/research/services/flow-cytometry/ ) Cell strainer filter (70 μm Nylon, Corning/Fisher Scientific, cat. no. 352350)

    Fluorescence:

    Article Title: A 3D human neural cell culture system for modeling Alzheimer's disease.
    Article Snippet: .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free microcentrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 thermal cycler (MJ Research, cat. no. ALD-1244) iCycler iQ (Bio-Rad, cat. no. 170-8740) Bio-Rad Tetrad 2 thermal cycler (Bio-Rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-Rad ChemiDoc XRS system (Bio-Rad, cat. no. 170-8070) IF and immunohistochemistry Olympus IX7 fluorescence microscope equipped with a spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical extraction and western blotting Hoefer semi-dry transfer unit (Hoefer, TE77) Molecular imager (Bio-Rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) • • • • • • • • • • • • • • • • • • • • • • • • Ultracentrifuge (Beckman, Optima TL ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) REAGENT SETUP Lentivirus We obtained the lentiviral polycistronic vectors (CSCW-IRES-GFP and CSCW-IRES-mCherry) from the Massachusetts General Hospital (MGH) viral core42, and these are available through the MGH viral core (https://vectorcore.mgh.harvard.edu). ..

    Article Title: A 3D human neural cell culture system for modeling Alzheimer’s disease
    Article Snippet: 0.4 μm disposable cell culture medium filter (Life Technologies, cat. no. 353095) Sterile 15 and 50 ml conical tube (Fisher Scientific/Falcon, cat. no. 352099 and 352070) Automated cell counter (Bio-rad, cat. no. TC10) Cell counter slide (Bio-rad, cat. no. 1450011) Water Bath (VWR Scientific, cat. no. 1202) Micro-centrifuge, 4 °C (Beckman Coulter, cat. no. 367160) Tissue culture Light microscope (Zeiss) BL-2 biosafety culture hoods Vortex Sorvall T6000B Centrifuge (Brochu) RNA extraction and RT-PCR Sorvall Legend T+ centrifuge (Thermo Scientific, cat. no. 75004367) RNaseZap®-rinsed, sterile forceps ▲ CRITICAL Forceps should be dedicated for RNA work only and stored in a separate RNA working area RNaseZap ® -rinsed, disposable razor blades RNase-free filter pipette tips (1, 20, 100, 1,000 μl; USA Scientific, cat. nos. .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free micro-centrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 Thermal Cycler (MJ Research, cat. no. ALD-1244) iCycler iQ TM (Bio-rad, cat. no. 170-8740) Bio-rad Tetrad® 2 Thermal Cycler (Bio-rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 Spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-rad ChemiDoc XRS System (Bio-rad, cat. no. 170-8070) Immunofluorescence and Immunohistochemistry Olympus IX7 fluorescence microscope equipped with spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical Extraction and Western Blot Hoefer Semi-Dry Transfer Unit (Hoefer, TE77) Molecular imager (Bio-rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) Ultracentrifuge (Beckman, Optima TL Ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader) .. We use a BD SORP FACSAria2 cell sorter (BD Bioscience) in a Bioprotect IV biosafety cabinet (Baker Company): equipped with 5 lasers (350, 405, 488, 561 and 640), located at the Imaging Core Facility of the Ragon Institute of MGH/MIT/Harvard ( http://ragoninstitute.org/research/services/flow-cytometry/ ) Cell strainer filter (70 μm Nylon, Corning/Fisher Scientific, cat. no. 352350)

    Microscopy:

    Article Title: A 3D human neural cell culture system for modeling Alzheimer's disease.
    Article Snippet: .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free microcentrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 thermal cycler (MJ Research, cat. no. ALD-1244) iCycler iQ (Bio-Rad, cat. no. 170-8740) Bio-Rad Tetrad 2 thermal cycler (Bio-Rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-Rad ChemiDoc XRS system (Bio-Rad, cat. no. 170-8070) IF and immunohistochemistry Olympus IX7 fluorescence microscope equipped with a spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical extraction and western blotting Hoefer semi-dry transfer unit (Hoefer, TE77) Molecular imager (Bio-Rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) • • • • • • • • • • • • • • • • • • • • • • • • Ultracentrifuge (Beckman, Optima TL ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) REAGENT SETUP Lentivirus We obtained the lentiviral polycistronic vectors (CSCW-IRES-GFP and CSCW-IRES-mCherry) from the Massachusetts General Hospital (MGH) viral core42, and these are available through the MGH viral core (https://vectorcore.mgh.harvard.edu). ..

    Article Title: A 3D human neural cell culture system for modeling Alzheimer’s disease
    Article Snippet: 0.4 μm disposable cell culture medium filter (Life Technologies, cat. no. 353095) Sterile 15 and 50 ml conical tube (Fisher Scientific/Falcon, cat. no. 352099 and 352070) Automated cell counter (Bio-rad, cat. no. TC10) Cell counter slide (Bio-rad, cat. no. 1450011) Water Bath (VWR Scientific, cat. no. 1202) Micro-centrifuge, 4 °C (Beckman Coulter, cat. no. 367160) Tissue culture Light microscope (Zeiss) BL-2 biosafety culture hoods Vortex Sorvall T6000B Centrifuge (Brochu) RNA extraction and RT-PCR Sorvall Legend T+ centrifuge (Thermo Scientific, cat. no. 75004367) RNaseZap®-rinsed, sterile forceps ▲ CRITICAL Forceps should be dedicated for RNA work only and stored in a separate RNA working area RNaseZap ® -rinsed, disposable razor blades RNase-free filter pipette tips (1, 20, 100, 1,000 μl; USA Scientific, cat. nos. .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free micro-centrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 Thermal Cycler (MJ Research, cat. no. ALD-1244) iCycler iQ TM (Bio-rad, cat. no. 170-8740) Bio-rad Tetrad® 2 Thermal Cycler (Bio-rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 Spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-rad ChemiDoc XRS System (Bio-rad, cat. no. 170-8070) Immunofluorescence and Immunohistochemistry Olympus IX7 fluorescence microscope equipped with spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical Extraction and Western Blot Hoefer Semi-Dry Transfer Unit (Hoefer, TE77) Molecular imager (Bio-rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) Ultracentrifuge (Beckman, Optima TL Ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader) .. We use a BD SORP FACSAria2 cell sorter (BD Bioscience) in a Bioprotect IV biosafety cabinet (Baker Company): equipped with 5 lasers (350, 405, 488, 561 and 640), located at the Imaging Core Facility of the Ragon Institute of MGH/MIT/Harvard ( http://ragoninstitute.org/research/services/flow-cytometry/ ) Cell strainer filter (70 μm Nylon, Corning/Fisher Scientific, cat. no. 352350)

    Extraction:

    Article Title: A 3D human neural cell culture system for modeling Alzheimer's disease.
    Article Snippet: .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free microcentrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 thermal cycler (MJ Research, cat. no. ALD-1244) iCycler iQ (Bio-Rad, cat. no. 170-8740) Bio-Rad Tetrad 2 thermal cycler (Bio-Rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-Rad ChemiDoc XRS system (Bio-Rad, cat. no. 170-8070) IF and immunohistochemistry Olympus IX7 fluorescence microscope equipped with a spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical extraction and western blotting Hoefer semi-dry transfer unit (Hoefer, TE77) Molecular imager (Bio-Rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) • • • • • • • • • • • • • • • • • • • • • • • • Ultracentrifuge (Beckman, Optima TL ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) REAGENT SETUP Lentivirus We obtained the lentiviral polycistronic vectors (CSCW-IRES-GFP and CSCW-IRES-mCherry) from the Massachusetts General Hospital (MGH) viral core42, and these are available through the MGH viral core (https://vectorcore.mgh.harvard.edu). ..

    Article Title: A 3D human neural cell culture system for modeling Alzheimer’s disease
    Article Snippet: 0.4 μm disposable cell culture medium filter (Life Technologies, cat. no. 353095) Sterile 15 and 50 ml conical tube (Fisher Scientific/Falcon, cat. no. 352099 and 352070) Automated cell counter (Bio-rad, cat. no. TC10) Cell counter slide (Bio-rad, cat. no. 1450011) Water Bath (VWR Scientific, cat. no. 1202) Micro-centrifuge, 4 °C (Beckman Coulter, cat. no. 367160) Tissue culture Light microscope (Zeiss) BL-2 biosafety culture hoods Vortex Sorvall T6000B Centrifuge (Brochu) RNA extraction and RT-PCR Sorvall Legend T+ centrifuge (Thermo Scientific, cat. no. 75004367) RNaseZap®-rinsed, sterile forceps ▲ CRITICAL Forceps should be dedicated for RNA work only and stored in a separate RNA working area RNaseZap ® -rinsed, disposable razor blades RNase-free filter pipette tips (1, 20, 100, 1,000 μl; USA Scientific, cat. nos. .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free micro-centrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 Thermal Cycler (MJ Research, cat. no. ALD-1244) iCycler iQ TM (Bio-rad, cat. no. 170-8740) Bio-rad Tetrad® 2 Thermal Cycler (Bio-rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 Spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-rad ChemiDoc XRS System (Bio-rad, cat. no. 170-8070) Immunofluorescence and Immunohistochemistry Olympus IX7 fluorescence microscope equipped with spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical Extraction and Western Blot Hoefer Semi-Dry Transfer Unit (Hoefer, TE77) Molecular imager (Bio-rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) Ultracentrifuge (Beckman, Optima TL Ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader) .. We use a BD SORP FACSAria2 cell sorter (BD Bioscience) in a Bioprotect IV biosafety cabinet (Baker Company): equipped with 5 lasers (350, 405, 488, 561 and 640), located at the Imaging Core Facility of the Ragon Institute of MGH/MIT/Harvard ( http://ragoninstitute.org/research/services/flow-cytometry/ ) Cell strainer filter (70 μm Nylon, Corning/Fisher Scientific, cat. no. 352350)

    Western Blot:

    Article Title: A 3D human neural cell culture system for modeling Alzheimer's disease.
    Article Snippet: .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free microcentrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 thermal cycler (MJ Research, cat. no. ALD-1244) iCycler iQ (Bio-Rad, cat. no. 170-8740) Bio-Rad Tetrad 2 thermal cycler (Bio-Rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-Rad ChemiDoc XRS system (Bio-Rad, cat. no. 170-8070) IF and immunohistochemistry Olympus IX7 fluorescence microscope equipped with a spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical extraction and western blotting Hoefer semi-dry transfer unit (Hoefer, TE77) Molecular imager (Bio-Rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) • • • • • • • • • • • • • • • • • • • • • • • • Ultracentrifuge (Beckman, Optima TL ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) REAGENT SETUP Lentivirus We obtained the lentiviral polycistronic vectors (CSCW-IRES-GFP and CSCW-IRES-mCherry) from the Massachusetts General Hospital (MGH) viral core42, and these are available through the MGH viral core (https://vectorcore.mgh.harvard.edu). ..

    Article Title: A 3D human neural cell culture system for modeling Alzheimer’s disease
    Article Snippet: 0.4 μm disposable cell culture medium filter (Life Technologies, cat. no. 353095) Sterile 15 and 50 ml conical tube (Fisher Scientific/Falcon, cat. no. 352099 and 352070) Automated cell counter (Bio-rad, cat. no. TC10) Cell counter slide (Bio-rad, cat. no. 1450011) Water Bath (VWR Scientific, cat. no. 1202) Micro-centrifuge, 4 °C (Beckman Coulter, cat. no. 367160) Tissue culture Light microscope (Zeiss) BL-2 biosafety culture hoods Vortex Sorvall T6000B Centrifuge (Brochu) RNA extraction and RT-PCR Sorvall Legend T+ centrifuge (Thermo Scientific, cat. no. 75004367) RNaseZap®-rinsed, sterile forceps ▲ CRITICAL Forceps should be dedicated for RNA work only and stored in a separate RNA working area RNaseZap ® -rinsed, disposable razor blades RNase-free filter pipette tips (1, 20, 100, 1,000 μl; USA Scientific, cat. nos. .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free micro-centrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 Thermal Cycler (MJ Research, cat. no. ALD-1244) iCycler iQ TM (Bio-rad, cat. no. 170-8740) Bio-rad Tetrad® 2 Thermal Cycler (Bio-rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 Spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-rad ChemiDoc XRS System (Bio-rad, cat. no. 170-8070) Immunofluorescence and Immunohistochemistry Olympus IX7 fluorescence microscope equipped with spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical Extraction and Western Blot Hoefer Semi-Dry Transfer Unit (Hoefer, TE77) Molecular imager (Bio-rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) Ultracentrifuge (Beckman, Optima TL Ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader) .. We use a BD SORP FACSAria2 cell sorter (BD Bioscience) in a Bioprotect IV biosafety cabinet (Baker Company): equipped with 5 lasers (350, 405, 488, 561 and 640), located at the Imaging Core Facility of the Ragon Institute of MGH/MIT/Harvard ( http://ragoninstitute.org/research/services/flow-cytometry/ ) Cell strainer filter (70 μm Nylon, Corning/Fisher Scientific, cat. no. 352350)

    Battery:

    Article Title: A 3D human neural cell culture system for modeling Alzheimer's disease.
    Article Snippet: .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free microcentrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 thermal cycler (MJ Research, cat. no. ALD-1244) iCycler iQ (Bio-Rad, cat. no. 170-8740) Bio-Rad Tetrad 2 thermal cycler (Bio-Rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-Rad ChemiDoc XRS system (Bio-Rad, cat. no. 170-8070) IF and immunohistochemistry Olympus IX7 fluorescence microscope equipped with a spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical extraction and western blotting Hoefer semi-dry transfer unit (Hoefer, TE77) Molecular imager (Bio-Rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) • • • • • • • • • • • • • • • • • • • • • • • • Ultracentrifuge (Beckman, Optima TL ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) REAGENT SETUP Lentivirus We obtained the lentiviral polycistronic vectors (CSCW-IRES-GFP and CSCW-IRES-mCherry) from the Massachusetts General Hospital (MGH) viral core42, and these are available through the MGH viral core (https://vectorcore.mgh.harvard.edu). ..

    Article Title: A 3D human neural cell culture system for modeling Alzheimer’s disease
    Article Snippet: 0.4 μm disposable cell culture medium filter (Life Technologies, cat. no. 353095) Sterile 15 and 50 ml conical tube (Fisher Scientific/Falcon, cat. no. 352099 and 352070) Automated cell counter (Bio-rad, cat. no. TC10) Cell counter slide (Bio-rad, cat. no. 1450011) Water Bath (VWR Scientific, cat. no. 1202) Micro-centrifuge, 4 °C (Beckman Coulter, cat. no. 367160) Tissue culture Light microscope (Zeiss) BL-2 biosafety culture hoods Vortex Sorvall T6000B Centrifuge (Brochu) RNA extraction and RT-PCR Sorvall Legend T+ centrifuge (Thermo Scientific, cat. no. 75004367) RNaseZap®-rinsed, sterile forceps ▲ CRITICAL Forceps should be dedicated for RNA work only and stored in a separate RNA working area RNaseZap ® -rinsed, disposable razor blades RNase-free filter pipette tips (1, 20, 100, 1,000 μl; USA Scientific, cat. nos. .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free micro-centrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 Thermal Cycler (MJ Research, cat. no. ALD-1244) iCycler iQ TM (Bio-rad, cat. no. 170-8740) Bio-rad Tetrad® 2 Thermal Cycler (Bio-rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 Spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-rad ChemiDoc XRS System (Bio-rad, cat. no. 170-8070) Immunofluorescence and Immunohistochemistry Olympus IX7 fluorescence microscope equipped with spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical Extraction and Western Blot Hoefer Semi-Dry Transfer Unit (Hoefer, TE77) Molecular imager (Bio-rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) Ultracentrifuge (Beckman, Optima TL Ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader) .. We use a BD SORP FACSAria2 cell sorter (BD Bioscience) in a Bioprotect IV biosafety cabinet (Baker Company): equipped with 5 lasers (350, 405, 488, 561 and 640), located at the Imaging Core Facility of the Ragon Institute of MGH/MIT/Harvard ( http://ragoninstitute.org/research/services/flow-cytometry/ ) Cell strainer filter (70 μm Nylon, Corning/Fisher Scientific, cat. no. 352350)

    Transmission Electron Microscopy:

    Article Title: A 3D human neural cell culture system for modeling Alzheimer's disease.
    Article Snippet: .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free microcentrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 thermal cycler (MJ Research, cat. no. ALD-1244) iCycler iQ (Bio-Rad, cat. no. 170-8740) Bio-Rad Tetrad 2 thermal cycler (Bio-Rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-Rad ChemiDoc XRS system (Bio-Rad, cat. no. 170-8070) IF and immunohistochemistry Olympus IX7 fluorescence microscope equipped with a spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical extraction and western blotting Hoefer semi-dry transfer unit (Hoefer, TE77) Molecular imager (Bio-Rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) • • • • • • • • • • • • • • • • • • • • • • • • Ultracentrifuge (Beckman, Optima TL ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) REAGENT SETUP Lentivirus We obtained the lentiviral polycistronic vectors (CSCW-IRES-GFP and CSCW-IRES-mCherry) from the Massachusetts General Hospital (MGH) viral core42, and these are available through the MGH viral core (https://vectorcore.mgh.harvard.edu). ..

    Article Title: A 3D human neural cell culture system for modeling Alzheimer’s disease
    Article Snippet: 0.4 μm disposable cell culture medium filter (Life Technologies, cat. no. 353095) Sterile 15 and 50 ml conical tube (Fisher Scientific/Falcon, cat. no. 352099 and 352070) Automated cell counter (Bio-rad, cat. no. TC10) Cell counter slide (Bio-rad, cat. no. 1450011) Water Bath (VWR Scientific, cat. no. 1202) Micro-centrifuge, 4 °C (Beckman Coulter, cat. no. 367160) Tissue culture Light microscope (Zeiss) BL-2 biosafety culture hoods Vortex Sorvall T6000B Centrifuge (Brochu) RNA extraction and RT-PCR Sorvall Legend T+ centrifuge (Thermo Scientific, cat. no. 75004367) RNaseZap®-rinsed, sterile forceps ▲ CRITICAL Forceps should be dedicated for RNA work only and stored in a separate RNA working area RNaseZap ® -rinsed, disposable razor blades RNase-free filter pipette tips (1, 20, 100, 1,000 μl; USA Scientific, cat. nos. .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free micro-centrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 Thermal Cycler (MJ Research, cat. no. ALD-1244) iCycler iQ TM (Bio-rad, cat. no. 170-8740) Bio-rad Tetrad® 2 Thermal Cycler (Bio-rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 Spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-rad ChemiDoc XRS System (Bio-rad, cat. no. 170-8070) Immunofluorescence and Immunohistochemistry Olympus IX7 fluorescence microscope equipped with spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical Extraction and Western Blot Hoefer Semi-Dry Transfer Unit (Hoefer, TE77) Molecular imager (Bio-rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) Ultracentrifuge (Beckman, Optima TL Ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader) .. We use a BD SORP FACSAria2 cell sorter (BD Bioscience) in a Bioprotect IV biosafety cabinet (Baker Company): equipped with 5 lasers (350, 405, 488, 561 and 640), located at the Imaging Core Facility of the Ragon Institute of MGH/MIT/Harvard ( http://ragoninstitute.org/research/services/flow-cytometry/ ) Cell strainer filter (70 μm Nylon, Corning/Fisher Scientific, cat. no. 352350)

    Transmission Assay:

    Article Title: A 3D human neural cell culture system for modeling Alzheimer's disease.
    Article Snippet: .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free microcentrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 thermal cycler (MJ Research, cat. no. ALD-1244) iCycler iQ (Bio-Rad, cat. no. 170-8740) Bio-Rad Tetrad 2 thermal cycler (Bio-Rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-Rad ChemiDoc XRS system (Bio-Rad, cat. no. 170-8070) IF and immunohistochemistry Olympus IX7 fluorescence microscope equipped with a spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical extraction and western blotting Hoefer semi-dry transfer unit (Hoefer, TE77) Molecular imager (Bio-Rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) • • • • • • • • • • • • • • • • • • • • • • • • Ultracentrifuge (Beckman, Optima TL ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) REAGENT SETUP Lentivirus We obtained the lentiviral polycistronic vectors (CSCW-IRES-GFP and CSCW-IRES-mCherry) from the Massachusetts General Hospital (MGH) viral core42, and these are available through the MGH viral core (https://vectorcore.mgh.harvard.edu). ..

    Article Title: A 3D human neural cell culture system for modeling Alzheimer’s disease
    Article Snippet: 0.4 μm disposable cell culture medium filter (Life Technologies, cat. no. 353095) Sterile 15 and 50 ml conical tube (Fisher Scientific/Falcon, cat. no. 352099 and 352070) Automated cell counter (Bio-rad, cat. no. TC10) Cell counter slide (Bio-rad, cat. no. 1450011) Water Bath (VWR Scientific, cat. no. 1202) Micro-centrifuge, 4 °C (Beckman Coulter, cat. no. 367160) Tissue culture Light microscope (Zeiss) BL-2 biosafety culture hoods Vortex Sorvall T6000B Centrifuge (Brochu) RNA extraction and RT-PCR Sorvall Legend T+ centrifuge (Thermo Scientific, cat. no. 75004367) RNaseZap®-rinsed, sterile forceps ▲ CRITICAL Forceps should be dedicated for RNA work only and stored in a separate RNA working area RNaseZap ® -rinsed, disposable razor blades RNase-free filter pipette tips (1, 20, 100, 1,000 μl; USA Scientific, cat. nos. .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free micro-centrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 Thermal Cycler (MJ Research, cat. no. ALD-1244) iCycler iQ TM (Bio-rad, cat. no. 170-8740) Bio-rad Tetrad® 2 Thermal Cycler (Bio-rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 Spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-rad ChemiDoc XRS System (Bio-rad, cat. no. 170-8070) Immunofluorescence and Immunohistochemistry Olympus IX7 fluorescence microscope equipped with spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical Extraction and Western Blot Hoefer Semi-Dry Transfer Unit (Hoefer, TE77) Molecular imager (Bio-rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) Ultracentrifuge (Beckman, Optima TL Ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader) .. We use a BD SORP FACSAria2 cell sorter (BD Bioscience) in a Bioprotect IV biosafety cabinet (Baker Company): equipped with 5 lasers (350, 405, 488, 561 and 640), located at the Imaging Core Facility of the Ragon Institute of MGH/MIT/Harvard ( http://ragoninstitute.org/research/services/flow-cytometry/ ) Cell strainer filter (70 μm Nylon, Corning/Fisher Scientific, cat. no. 352350)

    Imaging:

    Article Title: A 3D human neural cell culture system for modeling Alzheimer's disease.
    Article Snippet: .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free microcentrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 thermal cycler (MJ Research, cat. no. ALD-1244) iCycler iQ (Bio-Rad, cat. no. 170-8740) Bio-Rad Tetrad 2 thermal cycler (Bio-Rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-Rad ChemiDoc XRS system (Bio-Rad, cat. no. 170-8070) IF and immunohistochemistry Olympus IX7 fluorescence microscope equipped with a spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical extraction and western blotting Hoefer semi-dry transfer unit (Hoefer, TE77) Molecular imager (Bio-Rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) • • • • • • • • • • • • • • • • • • • • • • • • Ultracentrifuge (Beckman, Optima TL ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) REAGENT SETUP Lentivirus We obtained the lentiviral polycistronic vectors (CSCW-IRES-GFP and CSCW-IRES-mCherry) from the Massachusetts General Hospital (MGH) viral core42, and these are available through the MGH viral core (https://vectorcore.mgh.harvard.edu). ..

    Article Title: A 3D human neural cell culture system for modeling Alzheimer’s disease
    Article Snippet: 0.4 μm disposable cell culture medium filter (Life Technologies, cat. no. 353095) Sterile 15 and 50 ml conical tube (Fisher Scientific/Falcon, cat. no. 352099 and 352070) Automated cell counter (Bio-rad, cat. no. TC10) Cell counter slide (Bio-rad, cat. no. 1450011) Water Bath (VWR Scientific, cat. no. 1202) Micro-centrifuge, 4 °C (Beckman Coulter, cat. no. 367160) Tissue culture Light microscope (Zeiss) BL-2 biosafety culture hoods Vortex Sorvall T6000B Centrifuge (Brochu) RNA extraction and RT-PCR Sorvall Legend T+ centrifuge (Thermo Scientific, cat. no. 75004367) RNaseZap®-rinsed, sterile forceps ▲ CRITICAL Forceps should be dedicated for RNA work only and stored in a separate RNA working area RNaseZap ® -rinsed, disposable razor blades RNase-free filter pipette tips (1, 20, 100, 1,000 μl; USA Scientific, cat. nos. .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free micro-centrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 Thermal Cycler (MJ Research, cat. no. ALD-1244) iCycler iQ TM (Bio-rad, cat. no. 170-8740) Bio-rad Tetrad® 2 Thermal Cycler (Bio-rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 Spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-rad ChemiDoc XRS System (Bio-rad, cat. no. 170-8070) Immunofluorescence and Immunohistochemistry Olympus IX7 fluorescence microscope equipped with spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical Extraction and Western Blot Hoefer Semi-Dry Transfer Unit (Hoefer, TE77) Molecular imager (Bio-rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) Ultracentrifuge (Beckman, Optima TL Ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader) .. We use a BD SORP FACSAria2 cell sorter (BD Bioscience) in a Bioprotect IV biosafety cabinet (Baker Company): equipped with 5 lasers (350, 405, 488, 561 and 640), located at the Imaging Core Facility of the Ragon Institute of MGH/MIT/Harvard ( http://ragoninstitute.org/research/services/flow-cytometry/ ) Cell strainer filter (70 μm Nylon, Corning/Fisher Scientific, cat. no. 352350)

    Enzyme-linked Immunosorbent Assay:

    Article Title: A 3D human neural cell culture system for modeling Alzheimer's disease.
    Article Snippet: .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free microcentrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 thermal cycler (MJ Research, cat. no. ALD-1244) iCycler iQ (Bio-Rad, cat. no. 170-8740) Bio-Rad Tetrad 2 thermal cycler (Bio-Rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-Rad ChemiDoc XRS system (Bio-Rad, cat. no. 170-8070) IF and immunohistochemistry Olympus IX7 fluorescence microscope equipped with a spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical extraction and western blotting Hoefer semi-dry transfer unit (Hoefer, TE77) Molecular imager (Bio-Rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) • • • • • • • • • • • • • • • • • • • • • • • • Ultracentrifuge (Beckman, Optima TL ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) REAGENT SETUP Lentivirus We obtained the lentiviral polycistronic vectors (CSCW-IRES-GFP and CSCW-IRES-mCherry) from the Massachusetts General Hospital (MGH) viral core42, and these are available through the MGH viral core (https://vectorcore.mgh.harvard.edu). ..

    Article Title: A 3D human neural cell culture system for modeling Alzheimer’s disease
    Article Snippet: 0.4 μm disposable cell culture medium filter (Life Technologies, cat. no. 353095) Sterile 15 and 50 ml conical tube (Fisher Scientific/Falcon, cat. no. 352099 and 352070) Automated cell counter (Bio-rad, cat. no. TC10) Cell counter slide (Bio-rad, cat. no. 1450011) Water Bath (VWR Scientific, cat. no. 1202) Micro-centrifuge, 4 °C (Beckman Coulter, cat. no. 367160) Tissue culture Light microscope (Zeiss) BL-2 biosafety culture hoods Vortex Sorvall T6000B Centrifuge (Brochu) RNA extraction and RT-PCR Sorvall Legend T+ centrifuge (Thermo Scientific, cat. no. 75004367) RNaseZap®-rinsed, sterile forceps ▲ CRITICAL Forceps should be dedicated for RNA work only and stored in a separate RNA working area RNaseZap ® -rinsed, disposable razor blades RNase-free filter pipette tips (1, 20, 100, 1,000 μl; USA Scientific, cat. nos. .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free micro-centrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 Thermal Cycler (MJ Research, cat. no. ALD-1244) iCycler iQ TM (Bio-rad, cat. no. 170-8740) Bio-rad Tetrad® 2 Thermal Cycler (Bio-rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 Spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-rad ChemiDoc XRS System (Bio-rad, cat. no. 170-8070) Immunofluorescence and Immunohistochemistry Olympus IX7 fluorescence microscope equipped with spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical Extraction and Western Blot Hoefer Semi-Dry Transfer Unit (Hoefer, TE77) Molecular imager (Bio-rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) Ultracentrifuge (Beckman, Optima TL Ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader) .. We use a BD SORP FACSAria2 cell sorter (BD Bioscience) in a Bioprotect IV biosafety cabinet (Baker Company): equipped with 5 lasers (350, 405, 488, 561 and 640), located at the Imaging Core Facility of the Ragon Institute of MGH/MIT/Harvard ( http://ragoninstitute.org/research/services/flow-cytometry/ ) Cell strainer filter (70 μm Nylon, Corning/Fisher Scientific, cat. no. 352350)

    Software:

    Article Title: A 3D human neural cell culture system for modeling Alzheimer's disease.
    Article Snippet: .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free microcentrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 thermal cycler (MJ Research, cat. no. ALD-1244) iCycler iQ (Bio-Rad, cat. no. 170-8740) Bio-Rad Tetrad 2 thermal cycler (Bio-Rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-Rad ChemiDoc XRS system (Bio-Rad, cat. no. 170-8070) IF and immunohistochemistry Olympus IX7 fluorescence microscope equipped with a spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical extraction and western blotting Hoefer semi-dry transfer unit (Hoefer, TE77) Molecular imager (Bio-Rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) • • • • • • • • • • • • • • • • • • • • • • • • Ultracentrifuge (Beckman, Optima TL ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader powered by Gen5 analysis software) REAGENT SETUP Lentivirus We obtained the lentiviral polycistronic vectors (CSCW-IRES-GFP and CSCW-IRES-mCherry) from the Massachusetts General Hospital (MGH) viral core42, and these are available through the MGH viral core (https://vectorcore.mgh.harvard.edu). ..

    other:

    Article Title: A 3D human neural cell culture system for modeling Alzheimer’s disease
    Article Snippet: 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free micro-centrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 Thermal Cycler (MJ Research, cat. no. ALD-1244) iCycler iQ TM (Bio-rad, cat. no. 170-8740) Bio-rad Tetrad® 2 Thermal Cycler (Bio-rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 Spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-rad ChemiDoc XRS System (Bio-rad, cat. no. 170-8070) Immunofluorescence and Immunohistochemistry list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 Olympus IX7 fluorescence microscope equipped with spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical Extraction and Western Blot list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 Hoefer Semi-Dry Transfer Unit (Hoefer, TE77) Molecular imager (Bio-rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) Ultracentrifuge (Beckman, Optima TL Ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 ELISA plate reader (Bio-Tek Synergy 2 microplate reader) LDH list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 ELISA plate reader (Bio-Tek Synergy 2 microplate reader)

    Immunofluorescence:

    Article Title: A 3D human neural cell culture system for modeling Alzheimer’s disease
    Article Snippet: 0.4 μm disposable cell culture medium filter (Life Technologies, cat. no. 353095) Sterile 15 and 50 ml conical tube (Fisher Scientific/Falcon, cat. no. 352099 and 352070) Automated cell counter (Bio-rad, cat. no. TC10) Cell counter slide (Bio-rad, cat. no. 1450011) Water Bath (VWR Scientific, cat. no. 1202) Micro-centrifuge, 4 °C (Beckman Coulter, cat. no. 367160) Tissue culture Light microscope (Zeiss) BL-2 biosafety culture hoods Vortex Sorvall T6000B Centrifuge (Brochu) RNA extraction and RT-PCR Sorvall Legend T+ centrifuge (Thermo Scientific, cat. no. 75004367) RNaseZap®-rinsed, sterile forceps ▲ CRITICAL Forceps should be dedicated for RNA work only and stored in a separate RNA working area RNaseZap ® -rinsed, disposable razor blades RNase-free filter pipette tips (1, 20, 100, 1,000 μl; USA Scientific, cat. nos. .. 1121-3810, 1120-1810, 1120-8810 and 1126-7810, respectively) RNase-free micro-centrifuge tubes (1.5 ml size; USA Scientific, cat. no. 1615-5500) PTC-200 Thermal Cycler (MJ Research, cat. no. ALD-1244) iCycler iQ TM (Bio-rad, cat. no. 170-8740) Bio-rad Tetrad® 2 Thermal Cycler (Bio-rad, cat. no. PTC-0240G with ALS-1296GC) NanoDrop 1000 Spectrophotometer (Thermo Scientific, cat. no. ND-1000) Bio-rad ChemiDoc XRS System (Bio-rad, cat. no. 170-8070) Immunofluorescence and Immunohistochemistry Olympus IX7 fluorescence microscope equipped with spinning disk confocal unit (Olympus) LSM Pascal 5 Carl Zeiss confocal microscope (Zeiss) Biochemical Extraction and Western Blot Hoefer Semi-Dry Transfer Unit (Hoefer, TE77) Molecular imager (Bio-rad, VersaDoc MP4000) Battery-operated spinning homogenizer (MIDSCI, cat. no. A0001) Ultracentrifuge tubes (Beckman Coulter, cat. no. 343778) Ultracentrifuge (Beckman, Optima TL Ultracentrifuge) SpeedVac (Thermo Scientific, cat. no. SPD111V) Savant RVT4104 refrigerated vapor trap with Savant SC110 SpeedVac (Savant) TEM JEOL JEM 1011 transmission electron microscope (JEOL) AMT digital imaging system (Advanced Microscopy Techniques) ELISA ELISA plate reader (Bio-Tek Synergy 2 microplate reader) LDH ELISA plate reader (Bio-Tek Synergy 2 microplate reader) .. We use a BD SORP FACSAria2 cell sorter (BD Bioscience) in a Bioprotect IV biosafety cabinet (Baker Company): equipped with 5 lasers (350, 405, 488, 561 and 640), located at the Imaging Core Facility of the Ragon Institute of MGH/MIT/Harvard ( http://ragoninstitute.org/research/services/flow-cytometry/ ) Cell strainer filter (70 μm Nylon, Corning/Fisher Scientific, cat. no. 352350)



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